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mouse anti-exp2  (European Malaria Reagent)

 
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    European Malaria Reagent mouse anti-exp2
    Mouse Anti Exp2, supplied by European Malaria Reagent, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exp2/mouse+anti+exp2/pmc12011295-305-7-10
    Average 90 stars, based on 1 article reviews
    mouse anti-exp2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Malaria parasites use a soluble RhopH complex for erythrocyte invasion and an integral form for nutrient uptake
    Article Snippet: Proteins were separated on a 4–15% Mini-PROTEAN TGX gel (Bio-RAD) and transferred to nitrocellulose. .. After blocking, antibodies against CLAG3 (Nguitragool et al., 2011), Band3 (Santa Cruz), HA epi- tope tag (Sigma–Aldrich), EXP2 (European Malaria Reagent Repository), or aldolase (Abcam) were applied and visualized as described (Ito et al., 2017). .. Band intensities were quantified using ImageJ and analyzed in Prism (GraphPad).



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    Image Search Results


    Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

    Journal: Poultry Science

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    doi: 10.1016/j.psj.2025.106178

    Figure Lengend Snippet: Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

    Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

    Techniques: Virus, Control

    Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

    Journal: Poultry Science

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    doi: 10.1016/j.psj.2025.106178

    Figure Lengend Snippet: Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

    Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

    Techniques: Expressing, Vaccines, Comparison

    Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

    Journal: Poultry Science

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    doi: 10.1016/j.psj.2025.106178

    Figure Lengend Snippet: Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

    Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

    Techniques: HI Assay, Enzyme-linked Immunosorbent Assay, Virus, Comparison